Abstract B06: HTSvis: An user-friendly application for analysis of arrayed high-throughput experiments by interactive data representations

The presented application HTSvis provides an user-interface tool for interactive analysis of arrayed high-throughput screening experiments. Arrayed formats are broadly used for cell-based screens which help to reveal mechanisms of complex diseases such as cancer. Technological advances allow to screen tens of thousands experimental conditions in a single experiments resulting in large datasets with complicated annotation files. Software packages for standardized analysis with a minimized programming effort have been developed accordingly. Even though such pipelines provide comprehensive analysis options, interpretation of the results remains a challenge as the user has to manually compare files and possibly re-run the analysis to prosecute certain hypotheses. Here we present HTSvis, a web application that visualizes data from arrayed screens. The data can be read-in as result files of the established R package CellHTS2 or as any spreadsheet table. This enables the user to feed in data which has been statistically analyzed whilst preserving flexibility to analyze data independent of the CellHTS2 package. The application provides several informative data representations like heatmaps, scatter plots and tables. Within the application, the data can be browsed in an ad hoc manner via the implemented user interface. This allows the user to assess data quality, compare experiments, identify measurements of interest and to verify generated hypotheses in an exploratory manner. The dir...
Source: Molecular Cancer Therapeutics - Category: Cancer & Oncology Authors: Tags: New Technology and Bioinformatics: Poster Presentations - Proffered Abstracts Source Type: research