Topical tissue nano-transfection mediates non-viral stroma reprogramming and rescue
Nature Nanotechnology 12, 974 (2017).
doi:10.1038/nnano.2017.134
Authors: Daniel Gallego-Perez, Durba Pal, Subhadip Ghatak, Veysi Malkoc, Natalia Higuita-Castro, Surya Gnyawali, Lingqian Chang, Wei-Ching Liao, Junfeng Shi, Mithun Sinha, Kanhaiya Singh, Erin Steen, Alec Sunyecz, Richard Stewart, Jordan Moore, Thomas Ziebro, Robert G. Northcutt, Michael Homsy, Paul Bertani, Wu Lu, Sashwati Roy, Savita Khanna, Cameron Rink, Vishnu Baba Sundaresan, Jose J. Otero, L. James Lee & Chandan K. Sen
Although cellular therapies represent a promising strategy for a number of conditions, current approaches face major translational hurdles, including limited cell sources and the need for cumbersome pre-processing steps (for example, isolation, induced pluripotency). In vivo cell reprogramming has the potential to enable more-effective cell-based therapies by using readily available cell sources (for example, fibroblasts) and circumventing the need for ex vivo pre-processing. Existing reprogramming methodologies, however, are fraught with caveats, including a heavy reliance on viral transfection. Moreover, capsid size constraints and/or the stochastic nature of status quo approaches (viral and non-viral) pose additional limitations, thus highlighting the need for safer and more deterministic in vivo reprogramming methods. Here, we report a novel yet simple-to-implement non-viral approach to topically reprogram tissues through a nanochannelled device validated with well-establishe...
Source: Nature Nanotechnology - Category: Nanotechnology Authors: Daniel Gallego-Perez Durba Pal Subhadip Ghatak Veysi Malkoc Natalia Higuita-Castro Surya Gnyawali Lingqian Chang Wei-Ching Liao Junfeng Shi Mithun Sinha Kanhaiya Singh Erin Steen Alec Sunyecz Richard Stewart Jordan Moore Thomas Ziebro Robert G. Northcutt Tags: Letter Source Type: research