Characterization of a novel HLA ‐B*39:01:01‐related allele, HLA‐B*39:130, by cloning and phasing

Summary
A novel HLA‐B*39:01:01‐related variant, HLA‐B*39:130, has been identified in a normal individual of Han ethnicity in Hunan province, southern China. Following Sanger polymerase chain reaction–sequence‐based typing (PCR‐SBT), this new allele was further confirmed by cloning, phasing and sequencing. Aligned with HLA‐B*39:01:01, HLA‐B*39:130 has a nonsynonymous thymine substitution at nucleotide position 94 in exon 4, resulting in amino acid change from threonine to isoleucine at codon 214 (ACA→ATA) of the mature HLA‐BmRNA molecule.

Source: International Journal of Immunogenetics - October 1, 2017 Category: Genetics & Stem Cells Authors: L. X. Li, W. Tian, F. M. Zhu, W. Y. Wang, J. H. Cai Tags: SHORT COMMUNICATION Source Type: research