TGF ‐β pro‐oligodendrogenic effects on adult SVZ progenitor cultures and its interaction with the Notch signaling pathway

Abstract
Adult neural progenitor cells (NPCs) are capable of differentiating into neurons, astrocytes, and oligodendrocytes throughout life. Notch and transforming growth factor β1 (TGF‐β) signaling pathways play critical roles in controlling these cell fate decisions. TGF‐β has been previously shown to exert pro‐neurogenic effects on hippocampal and subventricular zone (SVZ) NPCs in vitro and to interact with Notch in different cellular types. Therefore, the aim of our work was to study the effect of TGF‐β on adult rat brain SVZ NPC glial commitment and its interaction with Notch signaling. Initial cell characterization revealed a large proportion of Olig2+, Nestin+, and glial fibrillary acidic protein (GFAP+) cells, a low percentage of platelet‐derived growth factor receptor α (PDGFRα+) or NG2+ cells, and <1% Tuj1+ cells. Immunocytochemical analyses showed a significant increase in the percentage of PDGFRα+, NG2+, and GFAP+ cells upon four‐day TGF‐β treatment, which demonstrates the pro‐gliogenic effect of this growth factor on adult brain SVZ NPCs. Real‐time polymerase chain reaction analyses showed that TGF‐β induced the expression of Notch ligand Jagged1 and downstream gene Hes1. Notch signaling inhibition in cultures treated with TGF‐β produced a decrease in the proportion of PDGFRα+ cells, while TGF‐β receptor II (TβRII) inhibition also rendered a decrease in the proportion of PDGFRα+ cells, concomitantly with a decrease in Jagg...

Source: Glia - October 27, 2017 Category: Neurology Authors: Laura I. G ómez Pinto, Debora Rodríguez, Ana M. Adamo, Patricia A. Mathieu Tags: RESEARCH ARTICLE Source Type: research