The diagnosis of microorganism involved in infective endocarditis (IE) by polymerase chain reaction (PCR) and real-time PCR: A systematic review

Publication date: Available online 24 October 2017 Source:The Kaohsiung Journal of Medical Sciences Author(s): Reza Faraji, Mostafa Behjati-Ardakani, Seyed Mohammad Moshtaghioun, Seyed Mehdi Kalantar, Seyedeh Mahdieh Namayandeh, Mohammadhossien Soltani, Mahmood Emami, Hengameh Zandi, Ali Dehghani Firoozabadi, Mahmood Kazeminasab, Nastaran Ahmadi, Mohammadtaghi Sarebanhassanabadi Broad-range bacterial rDNA polymerase chain reaction (PCR) followed by sequencing may be identified as the etiology of infective endocarditis (IE) from surgically removed valve tissue; therefore, we reviewed the value of molecular testing in identifying organisms' DNA in the studies conducted until 2016. We searched Google Scholar, Scopus, ScienceDirect, Cochrane, PubMed, and Medline electronic databases without any time limitations up to December 2016 for English studies reporting microorganisms involved in infective endocarditis microbiology using PCR and real-time PCR. Most studies were prospective. Eleven out of 12 studies used valve tissue samples and blood cultures while only 1 study used whole blood. Also, 10 studies used the molecular method of PCR while 2 studies used real-time PCR. Most studies used 16S rDNA gene as the target gene. The bacteria were identified as the most common microorganisms involved in infective endocarditis. Streptococcus spp. and Staphylococcus spp. were, by far, the most predominant bacteria detected. In all studies, PCR and real-time PCR identified more...
Source: The Kaohsiung Journal of Medical Sciences - Category: Universities & Medical Training Source Type: research