In vivo knockdown of antisense non ‐coding mitochondrial RNAs by a lentiviral‐encoded shRNA inhibits melanoma tumor growth and lung colonization
In this study, we knocked down the ASncmtRNAs in melanoma cell lines with a lentiviral‐encoded shRNA approach. Transduction with lentiviral constructs targeted to the ASncmtRNAs induced apoptosis in murine B16F10 and human A375 melanoma cells in vitro and significantly retarded B16F10 primary tumor growth in vivo. Moreover, the treatment drastically reduced the number of lung metastatic foci in a tail vein injection assay, compared to controls. These results provide additional proof of concept to the knockdown of ncmtRNAs for cancer therapy and validate lentiviral–shRNA vectors for gene therapy.
Source: Pigment Cell Research - Category: Cytology Authors: Manuel Varas ‐Godoy, Alvaro Lladser, Nicole Farfan, Claudio Villota, Jaime Villegas, Julio C. Tapia, Luis O. Burzio, Veronica A. Burzio, Pablo D. T. Valenzuela Tags: ORIGINAL ARTICLE Source Type: research
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