Modified RS ‐LAMP assay and use of lateral flow devices for rapid detection of Leifsonia xyli subsp. xyli

This study presents a modified loop‐mediated isothermal amplification (LAMP) assay for the detection of Leifsonia xyli subsp. xyli. Modifications include incorporation of uracil nucleoside glycosylase to eliminate carry‐over contamination and substitution of colorimetric detection for the use of lateral flow devices. LAMP master mix was preprepared and was stably stored up to 4 months at −20°C. Sugarcane leaf sheaths worked well as a substitute to xylem sap as template, although the sensitivity was lower. These modifications allow the assay to be conducted without contamination concerns and reduction in set up time, making it ideal for near‐field diagnosis.

Significance and Impact of the Study: This study presents a modified loop‐mediated isothermal amplification (LAMP) assay for the detection of Leifsonia xyli subsp. xyli. Modifications include incorporation of uracil nucleoside glycosylase to eliminate carry‐over contamination and substitution of colorimetric detection for the use of lateral flow devices. LAMP master mix was preprepared and was stably stored up to 4 months at −20°C. Sugarcane leaf sheaths worked well as a substitute to xylem sap as template, although the sensitivity was lower. These modifications allow the assay to be conducted without contamination concerns and reduction in set up time, making it ideal for near‐field diagnosis.

Source: Letters in Applied Microbiology - October 10, 2017 Category: Microbiology Authors: N. Naidoo, M. Ghai, K. Moodley, L. Mkize, L. Martin, S. McFarlane, S. Rutherford Tags: Original Article Source Type: research