Relevance of sample preparation for flow cytometry

ConclusionsWe suggest to use LWSW method also at diagnosis to obtain more comparable antibody intensity expressions when samples from the same patient are processed for MRD evaluation after bulk lysis. Moreover, LWSW can prevent nonspecific bindings, shows no differences in the identification and quantitation of the populations of interest, and reduces acquisition of cell debris.

Source: Clinical and Laboratory Haematology - October 6, 2017 Category: Hematology Authors: V. E. Muccio, E. Saraci, M. Gilestro, D. Oddolo, M. Ruggeri, S. Caltagirone, B. Bruno, M. Boccadoro, P. Omed è Tags: ORIGINAL ARTICLE Source Type: research