Ser71 of αS1‐Casein is Phosphorylated in Breast Milk—Evidence from Targeted Mass Analysis

ConclusionPhospho‐occupancy rates varied greatly and could not be confidently correlated to other parameters within the cohort of 20 donors. The new phosphosite S71 is located in the neighborhood of the serine‐rich region and may contribute to the cluster of high charge density at normal milk pH, likely exerting an influence on protein tertiary structure and thus function.

αS1‐casein phosphorylation at S71 is detected in breast milk by tandem mass spectrometry. The site is located in a sequences region different from that in other mammalian species but close to the conserved phosphoserine‐rich region. Phosphorylation at S71 influences the local negative charge density with an impact on protein tertiary structure.

Source: Nahrung / Food - October 4, 2017 Category: Nutrition Authors: Simone K önig, Irina Altendorfer, Thorsten Saenger, Ellen Bleck, Stefan Vordenbäumen, Matthias Schneider, Joachim Jose Tags: RESEARCH ARTICLE Source Type: research