The Resazurin Reduction Assay Can Distinguish Cytotoxic from Cytostatic Compounds in Spheroid Screening Assays

In conclusion, the RR assay is unsuited to quantitatively measure cellular health/cell number in compact spheroids. However, it can be used to distinguish between cytotoxic versus cytostatic compounds in spheroids. Restoration of the correlation of cell viability/number to resazurin reduction capacity can be achieved by disruption of tight junctions.

Source: Journal of Biomolecular Screening - July 18, 2014 Category: Molecular Biology Authors: Walzl, A., Unger, C., Kramer, N., Unterleuthner, D., Scherzer, M., Hengstschlager, M., Schwanzer-Pfeiffer, D., Dolznig, H. Tags: Original Research Source Type: research