Proteomic Identification of Synaptic Caspase Substrates

This article is protected by copyright. All rights reserved. The authors utilized a gel‐based proteomics approach to identify synaptic substrates of caspases, employing both an in vitro system with recombinant activated caspases as well as an in vivo model of ethanol‐induced neuronal apoptosis. Of the >70 putative cleavage substrates that were identified, 22 were previously known caspase substrates. The image depicts western blot validation of in vitro caspase 3 cleavage of N‐ethylmaleimide sensitive fusion protein (NSF), a key mediator of synaptic vesicle membrane fusion.
Source: Synapse - Category: Neurology Authors: Tags: Research Article Source Type: research