Bioactive effect of sulphated polysaccharides derived from orange-footed sea cucumber (Cucumaria frondosa) toward THP-1 macrophages

The objectives of this work were to test the immunomodulating properties of heteroglycan fractions (FCFs) derived from Atlantic sea cucumbers toward phorbol 12-myristate 13-acetate (PMA) differentiated THP-1 macrophages. Oxygen Radical Absorbance Capacity (ORAC) was determined and cell culture media was collected and analyzed for presence of pro-inflammatory and anti-inflammatory cytokines (TNF-α, IL-6, and IL-10). FCFs toxicity toward macrophages was evaluated by light microscopy and confirmed by XTT proliferation assay. Heteroglycans derived from cartilage of Atlantic sea cucumber showed immunomodulatory activity in THP-1 macrophages. FCF-1, −2, and − 3) at concentrations 0.1–100µg/ml induced levels of TNF-α, IL-6, and IL-10 proofing their bioactivity towards THP-1 macrophages. However, fractions of FCF-1 and FCF-2 may have induced oxidative stress in the macrophages, which possibly could lead to decreased cellular viability. Graphical abstract
Source: Bioactive Carbohydrates and Dietary Fibre - Category: Food Science Source Type: research