Endothelial marker-expressing stromal cells are critical for kidney formation

Kidneys are highly vascularized and contain many distinct vascular beds. However, the origins of renal endothelial cells and roles of the developing endothelia in the formation of the kidney are unclear. We have shown that the Foxd1-positive renal stroma gives rise to endothelial marker-expressing progenitors that are incorporated within a subset of peritubular capillaries; however, the significance of these cells is unclear. The purpose of this study was to determine whether deletion of Flk1 in the Foxd1 stroma was important for renal development. To that end, we conditionally deleted Flk1 (critical for endothelial cell development) in the renal stroma by breeding-floxed Flk1 mice (Flk1fl/fl) with Foxd1cre mice to generate Foxd1cre; Flk1fl/fl (Flk1ST–/–) mice. We then performed FACsorting, histological, morphometric, and metabolic analyses of Flk1ST–/– vs. control mice. We confirmed decreased expression of endothelial markers in the renal stroma of Flk1ST–/– kidneys via flow sorting and immunostaining, and upon interrogation of embryonic and postnatal Flk1ST–/– mice, we found they had dilated peritubular capillaries. Three-dimensional reconstructions showed reduced ureteric branching and fewer nephrons in developing Flk1ST–/– kidneys vs. controls. Juvenile Flk1ST–/– kidneys displayed renal papillary hypoplasia and a paucity of collecting ducts. Twenty-four-hour urine collections revealed that postnatal F...
Source: AJP: Renal Physiology - Category: Urology & Nephrology Authors: Tags: RESEARCH ARTICLE Source Type: research