Performance of the in vitro transgene mutation assay in MutaMouse FE1 cells: Evaluation of nine misleading ( “False”) positive chemicals

In this study, the performance of the FE1 cell assay was evaluated by examining responses to nine non‐DNA‐reactive chemicals that previously elicited misleading positive results in other mammalian cell genotoxicity assays. FE1 cells were exposed to concentrations up to approximately 10 mM and/or concentrations that yielded approximately 80‐90% cytotoxicity (as measured by relative increase in cell count). The assay demonstrated excellent specificity; exposures to the chemicals examined did not yield any positive responses even when tested in the presence of an exogenous metabolic activation system (i.e. S9) or with an extended sampling time. These results indicate that the FE1 cell mutagenicity assay is an effective and practical alternative to traditional mammalian cell gene mutation assays. The development and validation of effective in vitro tools such as the MutaMouse FE1 cell assay will contribute to international efforts to reduce, refine, and replace experimental animals for toxicity assessment. Environ. Mol. Mutagen., 2017. © 2017 Wiley Periodicals, Inc.

Source: Environmental and Molecular Mutagenesis - August 26, 2017 Category: Molecular Biology Authors: Rebecca M. Maertens, Alexandra S. Long, Paul A. White Tags: Research Article Source Type: research