CD103+ Dendritic Cell Function Is Altered in the Colons of Patients with Ulcerative Colitis

This study characterized CD103+ DCs in the colon and investigated whether these cells are implicated in the pathogenesis of ulcerative colitis (UC). Methods: Normal intestinal mucosa was obtained from intact sites of patients with colorectal cancer (n = 24). Noninflamed and inflamed colonic tissues were obtained from surgically resected specimens of patients with UC (n = 13). Among Lin−CD45+HLA-DRhigh intestinal lamina propria cells, CD14+ cells and CD103+ DCs were sorted and analyzed for microRNA expression of cytokines and toll-like receptors by quantitative real-time polymerase chain reaction. In addition, IL-4/IL-5/IL-13/IL-17/IFN-γ production and Foxp3 expression by naive T cells cultured with CD14+ cells and CD103+ DCs were analyzed. Results: CD103+ DCs in the normal colon showed lower expression of toll-like receptors and proinflammatory cytokines than CD14+ cells. Coculture with naive T cells revealed that CD103+ DCs generated Treg cells. CD103+ DCs from patients with UC did not generate Treg cells, but they induced IFN-γ-, IL-13-, and IL-17-producing CD4+ T cells and showed higher expression of IL6 (P
Source: Inflammatory Bowel Diseases - Category: Gastroenterology Tags: Original Basic Science Articles Source Type: research