Cold atmospheric plasma treatment affects early bacterial adhesion and decontamination of soft reline palatal obturators

Publication date: Available online 18 August 2017 Source:Clinical Plasma Medicine Author(s): Anna Liguori, Andrea Cochis, Augusto Stancampiano, Romolo Laurita, Barbara Azzimonti, Rita Sorrentino, Elena Varoni, Marta Petri, Vittorio Colombo, Matteo Gherardi, Lia Rimondini The aim of this work was to evaluate cold atmospheric plasma bactericide activity towards biofilm formation on soft reline oral palatal obturators. Plasma was generated using two dielectric barrier discharge and tested against the oral biofilm formers Streptococcus mutans and Aggregatibacter actinomycetemcomitans. Relines were either plasma treated before being infected with 24hour biofilm, or infected with 90minute (early) or 24hour (mature) biofilm prior to be plasma treated for 30, 60, or 120seconds. Bacteria numbers and viability were evaluated by Colonies Forming Units counts (CFU) and quantitative colorimetric metabolic 2,3-bis (2-methoxy-4-nitro-5-sulphophenyl)−5-[(phenyl amino) carbonyl]−2H-tetrazolium hydroxide assay (XTT). XTT assays. Plasma cytocompatibility was assayed towards human primary fibroblasts and keratinocytes cultivated on plasma-treated specimens by colorimetric metabolic 3-(4,5-Dimethylthiazol-2-Yl)−2,5-diphenyltetrazolium bromide (MTT) assay and light microscopy. Results demonstrated that both plasma sources were effective. Plasma treatment of sterile reline affected bacterial adhesion, reducing CFU number and viability by 2 logs and by 50%, in comparison with untr...

Source: Clinical Plasma Medicine - August 19, 2017 Category: Research Source Type: research