Quantitative Analysis of Ingenol in Euphorbia species via Validated Isotope Dilution Ultra ‐high Performance Liquid Chromatography Tandem Mass Spectrometry

ConclusionThe newly established UHPLC–MS/MS method shows to be an appropriate tool for screening of the Euphorbia genus for ingenol content and allows selection of species suitable for raw material production and/or in vitro culture initiation. Copyright © 2017 John Wiley & Sons, Ltd.

Ingenol is the key precursor for the synthesis of ingenol mebutate (angelate), used to treat actinic keratosis.
Various species of the Euphorbia genus contain diterpene ingenol and ingenol mebutate (ingenol‐3‐angelate), a substance found in the sap of the plant Euphorbia peplus and an inducer of cell death. An ultra‐high performance liquid chromatography tandem mass spectrometry‐based screening method suitable for quantitative analysis of ingenol in 38 species of the Euphorbia genus was developed. The 18O–labelled ingenol analogue was used as an internal standard for ingenol content determination and method validation.

Source: Phytochemical Analysis - July 1, 2017 Category: Biochemistry Authors: Tibor B éres, Klaus Dragull, Jiří Pospíšil, Danuše Tarkowská, Martin Dančák, Ondřej Bíba, Petr Tarkowski, Karel Doležal, Miroslav Strnad Tags: Research Article Source Type: research