Applied multiplex allele specific PCR to detect second-line drug resistance among multidrug-resistant tuberculosis in China
In this study, we applied a multiplex allele-specific PCR (MAS-PCR) to identify the mutations on codons 90 and 94 of gyrA and nucleotide 1401 of rrs for detecting ofloxacin (OFX) and kanamycin (KAN) resistance in 139 MDR-TB isolates from China. Using the traditional phenotypic method as the reference, MAS-PCR detected resistance to OFX and KAN with sensitivities of 67.3% and 76.5%, respectively, and specificities of 100.0%.
Source: Tuberculosis - Category: Respiratory Medicine Authors: Ya-ping Liang, Yan Chen, Tong-yang Xiao, Qiang Xia, Hai-can Liu, Xiu-qin Zhao, Chun-yan Zeng, Li-li Zhao, Kang-lin Wan Source Type: research