Influenza virus ‐like particles harboring H9N2 HA and NA proteins induce a protective immune response in chicken

ConclusionsThe hemagglutinin (HA) and neuraminidase (NA) proteins were co‐expressed in the infected cells, self‐assembled, and were released into the culture medium in the form of VLPs of diameter ~80 nm. The VLPs exhibited some functional characteristics of a full influenza virus, including hemagglutination and neuraminidase activity. In SPF chickens, the VLPs elicited serum antibodies specific for H9N2, and induced a higher HI titer (as detected by a homologous antigen) than did a commercial H9N2 vaccine (A/chicken/Shanghai/F/1998). Viral shedding from VLP vaccine sub‐group was reduced compared with commercial vaccine sub‐group and control sub‐group.This article is protected by copyright. All rights reserved.

Source: Influenza and Other Respiratory Viruses - July 1, 2017 Category: Virology Authors: Xin Li, Houbin Ju, Jian Liu, Dequan Yang, Xinyong Qi, Xianchao Yang, Yafeng Qiu, Jie Zheng, Feifei Ge, Jinping Zhou Tags: Original Article Source Type: research