SYCP3 regulates strand invasion activities of RAD51 and DMC1

In this study, we found that SYCP3 significantly suppresses the RAD51‐mediated, but not the DMC1‐mediated, strand invasion reaction by competing with HOP2‐MND1, which is an activator for both RAD51 and DMC1. A SYCP3 mutant with defective RAD51 binding does not inhibit the RAD51‐mediated homologous recombination in human cells. Therefore, SYCP3 may promote the DMC1‐driven homologous recombination by attenuating the RAD51 activity during meiosis.

SYCP3 is a component of the lateral/axial elements in the synaptonemal complex and is essential for meiotic recombination. We found that SYCP3 significantly suppresses the RAD51‐mediated, but not the DMC1‐mediated, strand invasion reaction in vitro.

Source: Genes to Cells - July 1, 2017 Category: Genetics & Stem Cells Authors: Wataru Kobayashi, Noriko Hosoya, Shinichi Machida, Kiyoshi Miyagawa, Hitoshi Kurumizaka Tags: Original Article Source Type: research