The role of prolactin in the suppression of Crh mRNA expression during pregnancy and lactation in the mouse

This study investigated the role of prolactin in regulating the basal activity of the HPA axis during pregnancy and lactation in the mouse, focussing upon the corticotropin‐releasing hormone (CRH) neurons. Using in situ hybridisation, a decrease in Crh mRNA‐expressing cell number in pregnant (55.6 ± 9.0 cells per section) and lactating (97.4 ± 4.9) mice in comparison to virgin controls was characterised (186.8 ± 18.7, p < 0.01 Tukey‐Kramer test; n = 6‐7 per group). Removal of the pups (24 h), and thus the associated suckling‐induced prolactin secretion, restored CRH neuron number (180.1 ± 19.7). To specifically test the role of prolactin in suppressing Crh mRNA expression in lactation, prolactin levels were selectively manipulated in lactating mice. Pup‐deprived lactating mice were treated with ovine prolactin (1500 μg/d, osmotic minipump, s.c.; n = 7) or vehicle (n = 6) for 24 h following pup removal. This was sufficient to suppress Crh mRNA expression from 108.0 ± 13.5 to 53.7 ± 16.7 cells per section (p < 0.05 Student's t‐test). Additional cohorts of lactating mice were treated with bromocriptine (300 μg over 24 h, s.c.; n = 7) or vehicle (n = 5) to suppress endogenous prolactin secretion, however no change in Crh mRNA expression was detected. Thus, while prolactin was sufficient to suppress Crh mRNA expression in the PVN, it does not appear to be required for the ongoing regulation of the CRH neurons in lactation. This article is protected by c...
Source: Journal of Neuroendocrinology - Category: Endocrinology Authors: Tags: Original Article Source Type: research