Mapping of Optimal CD8 T Cell Epitopes

Defining the optimal epitope of a CD8 T cell response towards a certain antigen is a multistep procedure that requires the performance of peptide truncation design, ELISPOT peptide titration assays, and assessing the HLA class I restriction of the defined epitope via intracellular cytokine staining assays with B cell lines and epitope-specific CD8 T cell lines.

Source: Springer protocols feed by Genetics/Genomics - June 24, 2014 Category: Genetics & Stem Cells Source Type: news