New, simple, sensitive and validated spectrophotometric method for the determination of salicylhydroxamic acid in capsules and raw material according to the ICH guidelines

Publication date: Available online 14 July 2017 Source:Egyptian Journal of Basic and Applied Sciences Author(s): Khaldun Mohammad Al Azzam, Wafaa El Kassed This work was aimed to develop a rapid, simple, selective, and precise spectrophotometric method for the estimation of salicylhydroxamic acid found in both capsules and raw materials. Spectrophotometric detection was conducted at maximum absorption of 294nm with the aids of methanol: water (1:99, v/v) as solvent. The figures of merit of the newly developed method were validated for linearity, specificity, accuracy, precision, robustness, ruggedness, limit of detection (LOD), and limit of quantification (LOQ). The detector response for the salicylhydroxamic acid was linear over the concentration range studied 0.1–50μgmL−1 with a correlation coefficient (R2) of 0.9999. Accuracy was between 99.0% and 101.7% with a mean value of 100.07%. The intra- and inter-day precisions, expressed as relative standard deviation (R.S.D.), were less than 0.0.322 and 0.421%, respectively. LOD and LOQ were 0.031 and 0.098μgmL−1, respectively. Results confirmed that the excipients in the commercial capsules did not interfere with the method and can be employed for routine quality control analysis of salicylhydroxamic acid whether in capsules or raw materials. Graphical abstract
Source: Egyptian Journal of Basic and Applied Sciences - Category: Science Source Type: research