Contributions of space-clamp errors to apparent time-dependent loss of Mg2+ block induced by NMDA

We report that upon sustained activation of NMDARs in juvenile mouse hippocampal neurons there is apparent loss of Mg2+ block at negative membrane potentials. However, the phenomenon is explained by loss of dendritic voltage clamp, leading to a linear current-voltage relationship. Our results give a specific example of how spatial voltage errors in voltage-clamp recordings can readily be misinterpreted as biological modulation.

Source: Journal of Neurophysiology - July 14, 2017 Category: Neurology Authors: Sun, M.-Y., Chisari, M., Eisenman, L. N., Zorumski, C. F., Mennerick, S. J. Tags: Research Articles Source Type: research