RGD peptide doped polypyrrole film as a biomimetic electrode coating for impedimetric sensing of cell proliferation and cytotoxicity

Publication date: Available online 12 July 2017 Source:Journal of Applied Biomedicine Author(s): Yuan Li, Chao Yu A novel and facile in vitro cell sensing system has been developed with one-step electropolymerization of the conducting polypyrrole(PPy) polymer using RGD peptide as the sole dopant on an indium tin oxide (ITO) surface. The resulted RGD peptide-doped polypyrrole (PPy/RGD) composite film had a robust surface, in which PPy provided a biocompatible matrix for cell growth and a conducting interface for electrical detection, while the RGD peptide entrapped in the PPy matrix conferred the desired biomimetic properties. Using the human lung cancer cell A549 as a model, this system can be used to monitor cell behaviors of proliferation and cytotoxicity. Graphical abstract
Source: Journal of Applied Biomedicine - Category: Biotechnology Source Type: research