Mammalian Cell Tissue Culture.

Mammalian Cell Tissue Culture.
Curr Protoc Hum Genet. 2017 Jul 11;94:A.3G.1-A.3G.22
Authors: Phelan K, May KM
Abstract
Cultured mammalian cells are used extensively in the field of human genetics. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2017 by John Wiley & Sons, Inc.
PMID: 28696558 [PubMed - in process]

Source: Current Protocols in Human Genetics - July 12, 2017 Category: Genetics & Stem Cells Tags: Curr Protoc Hum Genet Source Type: research