Super ‐Resolution Imaging of Subcortical White Matter using Stochastic Optical Reconstruction Microscopy (STORM) and Super‐Resolution Optical Fluctuation Imaging (SOFI)

ConclusionsSuper resolution imaging of intact cryo‐cut human brain tissue is feasible. For quantitation, STORM can under‐estimate diameters of thin fluorescent objects. SOFI is more robust. The greatest limitation for super‐resolution imaging in brain sections is imposed by sample preparation. We anticipate that improved strategies to reduce autofluorescence and to enhance fluorophore performance will enable rapid expansion of this approach. [232 words]This article is protected by copyright. All rights reserved.

Source: Neuropathology and Applied Neurobiology - July 11, 2017 Category: Neurology Authors: Atticus H Hainsworth, Sarah Lee, Peter Foot, Anita Patel, Wayne W Poon, Alex E Knight Tags: Original Article Source Type: research