Matrin3 binds directly to intronic pyrimidine ‐rich sequences and controls alternative splicing

In this study, we show that Matrin3, an RNA‐binding protein, binds predominantly to intronic regions of pre‐mRNAs. PAR‐CLIP analysis using human neuronal cells showed that Matrin3 recognized pyrimidine‐rich sequences as binding motifs, including the polypyrimidine tract, a splicing regulatory element. Depletion of Matrin3 preferentially increased the inclusion of cassette exons that were adjacent to introns that contained Matrin3‐binding sites, which suggests that Matrin3 is a splicing regulator.

Source: Genes to Cells - June 1, 2017 Category: Genetics & Stem Cells Authors: Yuri Uemura, Takuya Oshima, Munetaka Yamamoto, Charles Jourdan Reyes, Pedro Henrique Costa Cruz, Toshiharu Shibuya, Yukio Kawahara Tags: Original Article Source Type: research