FXYD5 (dysadherin) may mediate metastatic progression through regulation of the {beta}-Na+-K+-ATPase subunit in the 4T1 mouse breast cancer model

In this study, using in vivo 4T1 murine breast cancer model, we found that FXYD5-specific shRNA significantly inhibited lung cancer metastasis, without having a substantial effect on primary tumor growth. Our study reveals that FXYD5 participates in multiple stages of metastatic development and exhibits more than one mode of E-cadherin regulation. We provide the first evidence that FXYD5-related morphological changes are mediated through its interaction with Na+-K+-ATPase. Experiments in cultured 4T1 cells have indicated that FXYD5 expression may downregulate the β1 isoform of the pump. This behavior could have implications on both transcellular interactions and intracellular events. Further studies suggest that differential localization of the adaptor protein Annexin A2 in FXYD5-expressing cells may correlate with matrix metalloproteinase 9 secretion and adhesion changes in 4T1 wild-type cells.

Source: AJP: Cell Physiology - July 6, 2017 Category: Cytology Authors: Lubarski-Gotliv, I., Dey, K., Kuznetsov, Y., Kalchenco, V., Asher, C., Garty, H. Tags: RESEARCH ARTICLE Source Type: research