Influence of surface charge on the in vitro protein adsorption and cell cytotoxicity of paclitaxel loaded poly( ε-caprolactone) nanoparticles

Publication date: Available online 4 July 2017 Source:Bulletin of Faculty of Pharmacy, Cairo University Author(s): Sathyamoorthy Nandhakumar, Magharla Dasaratha Dhanaraju, Vankayalu Devendran Sundar, Battu Heera The biokinetic fate of polymeric nanoparticles in the physiological milieu is strongly influenced by its properties such as size, surface charge and surface affinity. The electrostatic properties of the polymeric nanoparticles and, thereby, the reliant properties such as cellular interactions, reactivity and toxicity, can be tailored by modulating the surface charge. Therefore, the present study aimed at studying the influence of surface charge on the physicochemical properties, in vitro protein adsorption and cell cytotoxicity of poly(ε-caprolactone) (PCL) nanoparticles (NPs). Paclitaxel loaded PCL nanoparticles were obtained by emulsion solvent evaporation extraction technique and differently charged using ionic surfactants. The NPs were characterized for size, zeta potential, morphology, entrapment and release. In vitro protein adsorption and cytotoxicity of NPs with different surface charge was investigated. The prepared NPs were rounded with a smooth surface and had a particle size less than 250nm with narrow distribution and high entrapment efficiency (>80%). The zeta potential of the particles varied between −22mV and +16mV depending on its composition. The in vitro protein adsorption studies revealed that positively charged NPs adsorbed more pr...
Source: Bulletin of Faculty of Pharmacy, Cairo University - Category: Drugs & Pharmacology Source Type: research