Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag

Easy-to-perform and low-cost protein purification methods are in high demand for the mass production of commonly used enzymes that play an important role in bioeconomy. A low-cost and rapid recombinant protein purification system was developed using CBM3 (family 3 cellulose-binding module) as affinity tag. This protocol describes the purification of CBM3-fusion protein and tag-free protein expressed in Pichia pastoris using CBM3 as an affinity tag.

Source: Springer protocols feed by Protein Science - June 19, 2014 Category: Biochemistry Source Type: news