A-type ECG and EGCG dimers inhibit 3 T3-L1 differentiation by binding to cholesterol in lipid rafts

The present study aimed to explore the underlying mechanisms of epicatechin-3-gallate-(4 β→8, 2β→O→7)-epicatechin-3-gallate (A-type ECG dimer) and epigallocatechin-3-gallate-(4β→8, 2β→O→7)-epigallocatechin-3-gallate (A-type EGCG dimer) involved in their strong inhibitory effects on 3 T3-L1 preadipocytes differentiation. In the synthetic “lipid raft-like” liposome, A- type ECG and EGCG dimers incorporated into the liposome with high affinity and decreased the fluidity of the liposome. In 3 T3-L1 preadipocytes, A-type ECG and EGCG dimers possibly bonded to lipid rafts cholesterol and disrupted the integrity of lipid rafts, thus exerting their notable inhibitory ef fects on 3 T3-L1 preadipocytes differentiation by suppressing mitotic clonal expansion process and mRNA levels of PPARγ, C/EBPα and SREBP1C.
Source: The Journal of Nutritional Biochemistry - Category: Biochemistry Authors: Source Type: research