Chlorogenic acid complex (CGA 7), standardized extract from green coffee beans exerts anticancer effects against cultured Human colon cancer HCT-116 cells

Publication date: Available online 21 June 2017 Source:Food Science and Human Wellness Author(s): K. Gouthamchandra, H.V. Sudeep, B.J. Venkatesh, K. Shyam Prasad Coffee is commonly consumed beverage in the world and it has been suggested to beneficial effect. Chlorogenic acids (CGAs) are main ingredient of coffee beans which has been extensively used in nutraceuticals and medicine. Recently, various therapeutic effects of chlorogenic acids have been investigated. However, there are limited studies to investigate its anticancer properties. In the present study, we have used chlorogenic acid complex (CGA7) a decaffeinated water soluble green coffee bean extract to evaluate its cytotoxic effect on human and mouse cancer cell lines by using different approaches. From our results we found CGA 7 treatment induces cell death in a dose and time dependent manner in different cancer cell lines. Further, CGA 7 induced apoptosis was characterized by DNA fragmentation, PARP-1 cleavage, caspase-9 activation, and down regulation of Bcl-2, an anti-apoptotic protein and up regulation of pro-apoptotic protein BAX. Overall findings indicated that CGA7 complex a potent anticancer molecule found in green coffee beans could be a safe bioactive ingredient for prevention of cancer.
Source: Food Science and Human Wellness - Category: Food Science Source Type: research