Stoichiometric analysis of oligomeric states of three class ‐A GPCRs, chemokine‐CXCR4, dopamine‐D2, and prostaglandin‐EP1 receptors, on living cells

In this study, we expanded our study to three well‐studied class‐A GPCRs: C‐X‐C chemokine receptor of stromal cell‐derived factor‐1α (CXCR4), dopamine receptor D2 short isotype (D2R), and prostaglandin E receptor subtype 1 (EP1R). We found that these receptors did not form constitutive homooligomers. The receptors exhibited calcium signaling upon agonist stimulation as monomers, although CXCR4 and EP1R gradually clustered after fast signaling. We conclude that homooligomerization is not necessary for the signal transductions of these four class‐A GPCRs. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd. Three well‐studied class‐A GPCRs, CXCR4, D2R, and EP1R, do not form constitutive homooligomers and exhibit calcium signaling upon agonist stimulation as monomers.
Source: Journal of Peptide Science - Category: Biochemistry Authors: Tags: Special Issue Article Source Type: research