Efficient determination of six fatty acid ethyl ethers in human whole blood by gas chromatography-mass spectrometry

Fatty acid ethyl esters (FAEEs) have been widely studied as specific markers of ethanol intake and mediators of ethanol-induced diseases. In the present study, a simple and rapid gas chromatography-mass spectrometry (GC-MS) method was established for the qualitative and quantitative analysis of six fatty acid ethyl esters (FAEEs), including ethyl myristate, ethyl palmitate, ethyl stearate, ethyl oleate, ethyl linoleate, and ethyl arachidonate, in human whole blood. FAEEs were extracted from 200 μL of human whole blood by a modified liquid-liquid extraction, and the hexane layer was injected directly into GC-MS with ethyl heptadecanoate as the internal standard.

Source: Alcohol - June 15, 2017 Category: Addiction Authors: Jiaolun Li, Xinyu Zhang, Zebin Lin, Zhibin Huang, Yulan Rao Source Type: research