Xenopus pitx3 target genes lhx1 and xnr5 are identified using a novel three ‐fluor flow cytometry‐based analysis of promoter activation and repression

Conclusions: We demonstrate how flow cytometry can be used to detect gene regulatory changes with exquisite precision on a cell‐by‐cell basis, and establish that in HEK293 cells, pitx3 directly activates lhx1 and represses xnr5. This article is protected by copyright. All rights reserved.

Source: Developmental Dynamics - June 9, 2017 Category: Molecular Biology Authors: Lara N. Hooker, Cristine Smoczer, Samuel Abbott, Mohamad Fakhereddin, John W. Hudson, Michael J. Crawford Tags: Research Article Source Type: research