Influence of extremely low frequency magnetic fields on Ca2+ signaling and double messenger system in mice hippocampus and reversal function of procyanidins extracted from lotus seedpod

This research investigated the influence of extremely low frequency magnetic fields (ELF‐MF; 50 Hz, 8 mT, 4 h per day, for 28 days) on calcium ion signaling and the double messenger system in the hippocampus of mice. Messengers that were studied included: G‐protein, Ins(1,4,5)P3 (IP3), diacylglycerol (DAG), cAMP‐dependent protein kinase (PKA), and Ca2+‐dependent protein kinase C (PKC). The results showed that ELF‐MF caused an increase in the levels of Gi protein, IP3, DAG, PKA and PKC beta, calcium and calmodulin‐dependent protein phosphatase calcineuring (PP2B), and intracellular Ca2+ content, and a decrease in calcium/calmodulin‐dependent protein kinase II (CaMK II) and PKC alpha. In addition, ELF‐MF exposure decreased the level of brain‐derived neurotrophic factor (BDNF), which played a key role in hippocampal neuronal cell death. However, oral administration of procyanidins from lotus seedpod (LSPCs) (especially 90 mg kg−1) significantly recovered these changes, and nearly reached normal levels. All these showed that LSPCs may mediate calcium signal and double messenger system through Ca2+/CaMK II/CREB/BDNF and DG/PKC/MAPK signaling pathways to reverse the alteration caused by ELF‐MF. Bioelectromagnetics. © 2017 Wiley Periodicals, Inc.

Source: Bioelectromagnetics - June 2, 2017 Category: Radiology Authors: Haihui Zhang, Yuanyuan Dai, Yanxiang Cheng, Yuanqing He, Zandile Manyakara, Yuqing Duan, Guibo Sun, Xiaobo Sun Tags: Research Article Source Type: research