Glycans in the intestinal peptide transporter PEPT1 contribute to function and protect from proteolysis

This study highlights the role of N50-linked glycans in modulating the bidirectional transport activity of the murine peptide transporter PEPT1. Electrophysiological and tracer flux measurements in Xenopus oocytes have shown that removal of the N50 glycans increases the maximal peptide transport rate in the inward and outward directions. This effect could be largely reversed by replacement of N50 glycans with structurally dissimilar biotin derivatives. In addition, N-glycans were detected to stabilize PEPT1 against proteolytic cleavage.

http://ajpgi.physiology.org/cgi/content/abstract/312/6/G580?rss=1

Source: AJP: Gastrointestinal and Liver Physiology - June 1, 2017 Category: Gastroenterology Authors: Stelzl, T., Geillinger-Kästle, K. E., Stolz, J., Daniel, H. Tags: RESEARCH ARTICLE Source Type: research