Ursodeoxycholic acid and lithocholic acid exert anti-inflammatory actions in the colon

Ward JB, Lajczak NK, Kelly OB, O’Dwyer AM, Giddam AK, Ní Gabhann J, Franco P, Tambuwala MM, Jefferies CA, Keely S, Roda A, Keely SJ. Ursodeoxycholic acid and lithocholic acid exert anti-inflammatory actions in the colon. Am J Physiol Gastrointest Liver Physiol 312: G550–G558, 2017. First published March 30, 2017; doi:10.1152/ajpgi.00256.2016.—Inflammatory bowel diseases (IBD) comprise a group of common and debilitating chronic intestinal disorders for which currently available therapies are often unsatisfactory. The naturally occurring secondary bile acid, ursodeoxycholic acid (UDCA), has well-established anti-inflammatory and cytoprotective actions and may therefore be effective in treating IBD. We aimed to investigate regulation of colonic inflammatory responses by UDCA and to determine the potential impact of bacterial metabolism on its therapeutic actions. The anti-inflammatory efficacy of UDCA, a nonmetabolizable analog, 6α-methyl-UDCA (6-MUDCA), and its primary colonic metabolite lithocholic acid (LCA) was assessed in the murine dextran sodium sulfate (DSS) model of mucosal injury. The effects of bile acids on cytokine (TNF-α, IL-6, Il-1β, and IFN-) release from cultured colonic epithelial cells and mouse colonic tissue in vivo were investigated. Luminal bile acids were measured by gas chromatography-mass spectrometry. UDCA attenuated release of proinflammatory cytokines from colonic epithelial cells in vitro and was protective ...

Source: AJP: Gastrointestinal and Liver Physiology - June 1, 2017 Category: Gastroenterology Authors: Ward, J. B. J., Lajczak, N. K., Kelly, O. B., ODwyer, A. M., Giddam, A. K., Ni Gabhann, J., Franco, P., Tambuwala, M. M., Jefferies, C. A., Keely, S., Roda, A., Keely, S. J. Tags: RESEARCH ARTICLE Source Type: research