Reverse Phase Protein Arrays elucidate mechanisms-of-action and phenotypic response in 2D and 3D models

We describe the application of Reverse Phase Protein Array (RPPA) technology to elucidate the mechanism-of-action of small molecules at the post-translational pathway level. We propose that profiling of phenotypic hits and lead molecules in increasingly more complex 3D in vitro and ex vivo models at the post-translational pathway network level represents an effective strategy to both triage and progress the preclinical development of phenotypic screening hits.
Source: Drug Discovery Today: Technologies - Category: Drugs & Pharmacology Source Type: research