In vitro site ‐specific recombination mediated by the tyrosine recombinase XerA of Thermoplasma acidophilum

In this study, we identified two XerA‐binding sites (dif1 and dif2) in the Thermoplasma acidophilum chromosome. In vitro site‐specific recombination assays showed that dif2, but not dif1, serves as a target site for XerA‐mediated chromosome resolution. Mutational analysis indicated that not only the core consensus sequence of dif2, but also its flanking regions play important roles in the recognition and recombination reactions mediated by XerA. XerA resolves a plasmid dimer with the Peak 2 sequence to monomers.
Source: Genes to Cells - Category: Genetics & Stem Cells Authors: Tags: Original Article Source Type: research