Recombinant form of mammalian gp91phox is active in the absence of p22phox

In this study, we have generated in Pichia pastoris for the first time an active form of bovine gp91phox able to carry out the entire NADPH oxidase activity in the absence of p22phox. Collected information on the maturation and the activity of the recombinant gp91phox and the participation of individual cytosolic subunits in the active complex allowed us also to propose, in the absence of p22phox, an unconventional stabilized complex compared to the heterodimer.

Source: BJ Cell - June 3, 2014 Category: Biochemistry Authors: A Ezzine, H Souabni, T Bizouarn, L Baciou Tags: BJ Biomolecules Source Type: research