Body mass index is associated with region-dependent metabolic reprogramming of adipose tissue

Publication date: Available online 11 May 2017 Source:BBA Clinical Author(s): Marco G. Alves, Ângela Moreira, Marta Guimarães, Mário Nora, Mario Sousa, Pedro F. Oliveira, Mariana P. Monteiro Adipose tissue (AT) is involved in dysmetabolism pathogenesis. Regional fat distribution and functioning may contribute to obesity-related metabolic disorders and adverse health outcomes. Specific fat depots are suggested to possess unique biological properties, but specific metabolic profiles of subcutaneous (SAT) and visceral adipose tissue (VAT) remain unknown. We aimed to characterize VAT and SAT glucose metabolism, and their correlation with body mass index (BMI). AT samples from patients (n =12; F:M, 9:3) with a mean age of 46years (26–83years) and an average BMI of 29.6kg/m2 (18–37kg/m2) were used. VAT and SAT explants were obtained during elective laparoscopy, either cholecystectomy for uncomplicated cholelithiasis or gastric bypass for severe obesity. Explants were placed in insulin-free cell culture media and their metabolic profile was established by proton nuclear magnetic resonance. AT explants display a glucose and pyruvate consumption and acetate production that is region-dependent according with the patients BMI. In VAT, glucose consumption was positively correlated with BMI, while alanine and lactate production were negatively correlated with BMI, whereas in SAT the patients BMI did not affect AT secretome suggesting that increased BMI promotes a metabolic...
Source: BBA Clinical - Category: Biochemistry Source Type: research