Effect of phyto-synthesized silver nanoparticles on developmental stages of malaria vector, Anopheles stephensi and dengue vector, Aedes aegypti

Publication date: Available online 11 May 2017 Source:Egyptian Journal of Basic and Applied Sciences Author(s): Madanagopal Nalini, Mahalingam Lena, Palanisamy Sumathi, Chandran Sundaravadivelan Rapidly synthesized phyto-mediated silver nanoparticles (Ag NPs) using Artemisia nilagirica aqueous leaf filtrate has been confirmed through UV–visible spectrophotometer. The synthesized Ag NPs were further characterized using Fourier transform infra-red (FTIR), X-ray diffraction analysis (XRD) to determine the present of functional groups and average particle size (6.723nm with cubic nature), respectively. Spherical shape (≤30nm) of Ag NPs was confirmed by scanning electron microscopy (SEM). Bio-efficacy of these nanoparticles showed larvicidal and pupicidal properties than the aqueous leaf extract treatment alone against developmental stages (I–IV instars and pupa) of malaria vector Anopheles stephensi and dengue vector Aedes aegypti at 0.25% concentration level. The LC50 (LCL:UCL at 95% confidence limit) values of I–IV instar and pupa of An. stephensi were recorded at 0.343 (0.261:0.405), 0.169 (0.025:0.263), 0.198 (0.105:0.265), 0.141 (0.045:0.205) and 0.050 (0.606:0.224) % respectively and for Ae. aegypti (I–IV instar and pupa) 0.460 (0.364:0.537), 0.352 (0.239:0.432), 0.331 (0.833:0.549), 0.217 (0.228:0.378) and 0.161 (0.630:0.356) % were observed, after 24h exposure. The first report of present investigation revealed that the rapid biological synthesis of silver...
Source: Egyptian Journal of Basic and Applied Sciences - Category: Science Source Type: research