Effects of non-glycosylated and glycosylated prolactin on basal and gonadotropin-stimulated steroidogenesis in chicken ovarian follicles

In galliformes, the circulating isoform of prolactin (PRL) significantly changes during different reproductive states. However, the role of the major isoform (glycosylated PRL, G-PRL) in ovarian steroidogenesis is unknown. The present study aimed to compare the effects of non-glycosylated (NG-) and G-PRL on basal and gonadotropin-stimulated estradiol (E2) and progesterone (P4) production in granulosa cells or follicular walls of chicken different size class follicles. In the initial experiment, granulosa cells of preovulatory F3-F1 and prehierarchical 6-8 mm follicles were incubated for 24 with different concentrations of NG- or G-PRL (0,1 ,10 ,100 or 1000 ng/mL).
Source: Domestic Animal Endocrinology - Category: Endocrinology Authors: Source Type: research