Assessment of treatment response by colony forming units, time to culture positivity and the molecular bacterial load assay compared in a mouse tuberculosis model

The aim of the study is to compare counting of colony forming units (CFU), the time to positivity (TTP) assay and the molecular bacterial load (MBL) assay, and explore whether the last assays can detect a subpopulation which is unable to grown on solid media. CFU counting, TTP and the MBL assay were used to determine the mycobacterial load in matched lung samples of a murine tuberculosis model. Mice were treated for 24 weeks with 4 treatment arms: isoniazid (H) - rifampicin (R) - pyrazinamide (Z), HRZ-Streptomycin (S), HRZ – ethambutol (E) or ZES.

Source: Tuberculosis - May 8, 2017 Category: Respiratory Medicine Authors: Gerjo J. de Knegt, Laura Dickinson, Henry Pertinez, Dimitrios Evangelopoulos, Timothy D. McHugh, Irma A.J.M. Bakker-Woudenberg, Gerry R. Davies, Jurriaan E.M. de Steenwinkel Source Type: research