Using FunSeq2 for Coding and Non-Coding Variant Annotation and Prioritization.

Using FunSeq2 for Coding and Non-Coding Variant Annotation and Prioritization. Curr Protoc Bioinformatics. 2017 May 02;57:15.11.1-15.11.17 Authors: Dhingra P, Fu Y, Gerstein M, Khurana E Abstract The identification of non-coding drivers remains a challenge and bottleneck for the use of whole-genome sequencing in the clinic. FunSeq2 is a computational tool for annotation and prioritization of somatic mutations in coding and non-coding regions. It integrates a data context made from large-scale genomic datasets and uses a high-throughput variant prioritization pipeline. This unit provides guidelines for installing and running FunSeq2 to (a) annotate and prioritize variants, (b) incorporate user-defined annotations, and (c) detect differential gene expression. © 2017 by John Wiley & Sons, Inc. PMID: 28463398 [PubMed - in process]
Source: Current Protocols in Bioinformatics - Category: Bioinformatics Tags: Curr Protoc Bioinformatics Source Type: research