In-vitro antioxidant, Xanthine oxidase-inhibitory and in-vivo Anti-inflammatory, analgesic, antipyretic activity of < em > Onopordum acanthium < /em >

Onopordum acanthium (Scotch thistle) belong to Asteraceae (Compositae).O. acanthium is a flowering biennial plant native to Europe and Western Asia with coarse spiny leaves 20-50 cm in width with conspicuous and spiny-winged stems. We have previously reported pro-apoptotic and cytotoxic effect ofOnopordum acanthium crude extract against glioblastoma U-373 cells. The present study was designed to evaluate the cytotoxicity, antioxidant, xanthine oxidase inhibition, anti-inflammatory, analgesic, antipyretic activity of butanolic extract ofOnopordum acanthium. Cytotoxicity of different solvent (methanolic, butanol, chloroform and petroleum ether) extract studied by brine shrimp lethality bioassay, total flavonoid and phenolic, antioxidant, xanthine oxidase inhibition activity was studied byin-vitro whereas anti- inflammation studied by carrageenan-induced paw edema model, antipyretic with 20 % brew yeast injection induced pyretic model, analgesic with 1 % acetic acid induced analgesic model investigated inin-vivoin wistar rats. Good antioxidant activity was found with IC50 = 134.4 µg/ml with considerable amount of total phenolic and flavonoid content. Xanthine oxidase inhibition effect was weak with IC50 = 572.9 µg/ml. Oral administration ofO. Acanthium butanolic extract (OA) showed minimum lethality of brine shrimp nauplii henceforth OA butanolic phases was selected for furtherin-vivo studies. OA 200 and 400 mg/kg body weight decreased the oedema by 37.78 % and 40.52 %, respec...

Source: International Journal of Phytomedicine - April 30, 2017 Category: Science Authors: Sofiane Habibatni Source Type: research