Alzheimer´s Disease-associated Aβ42 Peptide: Expression and Purification for NMR Structural Studies

Conclusion: This approach, which does not require the use of costly and time-consuming reversed phase high performance liquid chromatography (RP-HPLC), offers a much straightforward strategy to those previously described to obtain [U-15N] Aβ42 and it is the first protocol through which to achieve [U-2H,13C,15N] Aβ42. The peptides obtained are of high purity and have the required isotope enrichment to support NMR-based structural studies.
Source: Current Chemical Biology - Category: Biochemistry Source Type: research