Trimming of glucosylated N-glycans by human ER {alpha}1,2-mannosidase I

In this study, we revealed that this enzyme also accepts N-linked sugar chains with glucose moieties as substrates with nearly identical reactivity. The ability of hERManI to remove mannose residues from GlcMan9GlcNAc2 in model glycoproteins, such as Aspergillus oryzae β-galactosidase and chicken immunoglobulin Y (IgY), was markedly augmented when glycoproteins were denatured. The properties of hERManI enable rapid selection of ERAD substrates in the ER and may help maintain homeostasis of sugar metabolism in living organisms.

Source: Journal of Biochemistry - May 27, 2014 Category: Biochemistry Authors: Aikawa, J.-i., Takeda, Y., Matsuo, I., Ito, Y. Tags: Regular Papers Source Type: research